rap1 activation assay kit Search Results


active rap1 detection kit  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc active rap1 detection kit
GDF6 activates AMPKα through cyclic AMP/exchange protein directly activated by cAMP 1 (cAMP/Epac1) pathway. ( A ) The levels of cAMP and protein kinase A (PKA) activity in PE-stimulated NRVMs with or without GDF6 knockdown. ( B ) The levels of cAMP and PKA activity in PE-stimulated NRVMs with or without rhGDF6 treatment. ( C ) Quantification of cell area in rhGDF6-treated NRVMs with or without adenylyl cyclase (AC) inhibition. ( D ) <t>Rap1-GTP</t> and total Rap1 protein levels in PE-stimulated NRVMs treated with si Gdf6 or rhGDF6. ( E ) AMPKα protein levels in PE-stimulated NRVMs with or without Epac1 silence in the presence or absence of rhGDF6. ( F ) Quantification of cell area. ( G ) Epac1 mRNA levels in NRVMs with or without Epac1 silence. ( H ) Quantification of cell area. ( I ) Epac1 mRNA levels in murine hearts with or without Epac1 silence. ( J ) HW/TL in TAC mice with or without Epac1 silence in the presence or absence of AAV9- Gdf6 . ( K ) Quantification of CSA. ( L ) IVSd and IVSs. ( M ) FS, LVEDd, and LVEDs. n = 6 per group. * p < 0.05 versus matched AAV9- Ctrl -injected TAC mice receiving si Ctrl injection, # p < 0.05 versus matched AAV9- Gdf6 -injected TAC mice receiving si Ctrl injection. In ( A – D , G – I ), * p < 0.05 versus the matched groups. In ( E , F ), * p < 0.05 versus matched si Ctrl -transfected NRVMs with DMSO treatment under PE incubation, # p < 0.05 versus matched si Ctrl -transfected NRVMs with rhGDF6 treatment under PE incubation. One-way ANOVA followed by Tukey post hoc test was applied. In ( A , B , D , G – I ), Student’s two-tailed t -test was performed.
Active Rap1 Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/active rap1 detection kit/product/Cell Signaling Technology Inc
Average 93 stars, based on 1 article reviews
active rap1 detection kit - by Bioz Stars, 2026-06
93/100 stars
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anti-gtp-rap1 antibody  (NewEast Biosciences)
90
NewEast Biosciences anti-gtp-rap1 antibody
GDF6 activates AMPKα through cyclic AMP/exchange protein directly activated by cAMP 1 (cAMP/Epac1) pathway. ( A ) The levels of cAMP and protein kinase A (PKA) activity in PE-stimulated NRVMs with or without GDF6 knockdown. ( B ) The levels of cAMP and PKA activity in PE-stimulated NRVMs with or without rhGDF6 treatment. ( C ) Quantification of cell area in rhGDF6-treated NRVMs with or without adenylyl cyclase (AC) inhibition. ( D ) <t>Rap1-GTP</t> and total Rap1 protein levels in PE-stimulated NRVMs treated with si Gdf6 or rhGDF6. ( E ) AMPKα protein levels in PE-stimulated NRVMs with or without Epac1 silence in the presence or absence of rhGDF6. ( F ) Quantification of cell area. ( G ) Epac1 mRNA levels in NRVMs with or without Epac1 silence. ( H ) Quantification of cell area. ( I ) Epac1 mRNA levels in murine hearts with or without Epac1 silence. ( J ) HW/TL in TAC mice with or without Epac1 silence in the presence or absence of AAV9- Gdf6 . ( K ) Quantification of CSA. ( L ) IVSd and IVSs. ( M ) FS, LVEDd, and LVEDs. n = 6 per group. * p < 0.05 versus matched AAV9- Ctrl -injected TAC mice receiving si Ctrl injection, # p < 0.05 versus matched AAV9- Gdf6 -injected TAC mice receiving si Ctrl injection. In ( A – D , G – I ), * p < 0.05 versus the matched groups. In ( E , F ), * p < 0.05 versus matched si Ctrl -transfected NRVMs with DMSO treatment under PE incubation, # p < 0.05 versus matched si Ctrl -transfected NRVMs with rhGDF6 treatment under PE incubation. One-way ANOVA followed by Tukey post hoc test was applied. In ( A , B , D , G – I ), Student’s two-tailed t -test was performed.
Anti Gtp Rap1 Antibody, supplied by NewEast Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-gtp-rap1 antibody/product/NewEast Biosciences
Average 90 stars, based on 1 article reviews
anti-gtp-rap1 antibody - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
active rap1 pull down and detection kit  (Thermo Fisher)
N/A
The Thermo Scientific Pierce Active Rap1 Pull Down and Detection Kit is a complete kit for selective enrichment and detection of GTP bound Rap1 GTPase through specific protein interaction with the RalGDS protein binding domain
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human rap1 terf2ip activation kit by crispra  (OriGene)
N/A
TERF2IP CRISPRa kit CRISPR gene activation of human TERF2 interacting protein
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Image Search Results


GDF6 activates AMPKα through cyclic AMP/exchange protein directly activated by cAMP 1 (cAMP/Epac1) pathway. ( A ) The levels of cAMP and protein kinase A (PKA) activity in PE-stimulated NRVMs with or without GDF6 knockdown. ( B ) The levels of cAMP and PKA activity in PE-stimulated NRVMs with or without rhGDF6 treatment. ( C ) Quantification of cell area in rhGDF6-treated NRVMs with or without adenylyl cyclase (AC) inhibition. ( D ) Rap1-GTP and total Rap1 protein levels in PE-stimulated NRVMs treated with si Gdf6 or rhGDF6. ( E ) AMPKα protein levels in PE-stimulated NRVMs with or without Epac1 silence in the presence or absence of rhGDF6. ( F ) Quantification of cell area. ( G ) Epac1 mRNA levels in NRVMs with or without Epac1 silence. ( H ) Quantification of cell area. ( I ) Epac1 mRNA levels in murine hearts with or without Epac1 silence. ( J ) HW/TL in TAC mice with or without Epac1 silence in the presence or absence of AAV9- Gdf6 . ( K ) Quantification of CSA. ( L ) IVSd and IVSs. ( M ) FS, LVEDd, and LVEDs. n = 6 per group. * p < 0.05 versus matched AAV9- Ctrl -injected TAC mice receiving si Ctrl injection, # p < 0.05 versus matched AAV9- Gdf6 -injected TAC mice receiving si Ctrl injection. In ( A – D , G – I ), * p < 0.05 versus the matched groups. In ( E , F ), * p < 0.05 versus matched si Ctrl -transfected NRVMs with DMSO treatment under PE incubation, # p < 0.05 versus matched si Ctrl -transfected NRVMs with rhGDF6 treatment under PE incubation. One-way ANOVA followed by Tukey post hoc test was applied. In ( A , B , D , G – I ), Student’s two-tailed t -test was performed.

Journal: Biomedicines

Article Title: GDF6 Alleviates Pathological Cardiac Hypertrophy via AMPKα Signaling Pathway

doi: 10.3390/biomedicines13122935

Figure Lengend Snippet: GDF6 activates AMPKα through cyclic AMP/exchange protein directly activated by cAMP 1 (cAMP/Epac1) pathway. ( A ) The levels of cAMP and protein kinase A (PKA) activity in PE-stimulated NRVMs with or without GDF6 knockdown. ( B ) The levels of cAMP and PKA activity in PE-stimulated NRVMs with or without rhGDF6 treatment. ( C ) Quantification of cell area in rhGDF6-treated NRVMs with or without adenylyl cyclase (AC) inhibition. ( D ) Rap1-GTP and total Rap1 protein levels in PE-stimulated NRVMs treated with si Gdf6 or rhGDF6. ( E ) AMPKα protein levels in PE-stimulated NRVMs with or without Epac1 silence in the presence or absence of rhGDF6. ( F ) Quantification of cell area. ( G ) Epac1 mRNA levels in NRVMs with or without Epac1 silence. ( H ) Quantification of cell area. ( I ) Epac1 mRNA levels in murine hearts with or without Epac1 silence. ( J ) HW/TL in TAC mice with or without Epac1 silence in the presence or absence of AAV9- Gdf6 . ( K ) Quantification of CSA. ( L ) IVSd and IVSs. ( M ) FS, LVEDd, and LVEDs. n = 6 per group. * p < 0.05 versus matched AAV9- Ctrl -injected TAC mice receiving si Ctrl injection, # p < 0.05 versus matched AAV9- Gdf6 -injected TAC mice receiving si Ctrl injection. In ( A – D , G – I ), * p < 0.05 versus the matched groups. In ( E , F ), * p < 0.05 versus matched si Ctrl -transfected NRVMs with DMSO treatment under PE incubation, # p < 0.05 versus matched si Ctrl -transfected NRVMs with rhGDF6 treatment under PE incubation. One-way ANOVA followed by Tukey post hoc test was applied. In ( A , B , D , G – I ), Student’s two-tailed t -test was performed.

Article Snippet: Active Rap1 Detection Kit (#8818) was purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Activity Assay, Knockdown, Inhibition, Injection, Transfection, Incubation, Two Tailed Test